PCR artifacts in LOH and MSI analysis of microdissected tumor cells.

@article{Sieben2000PCRAI,
  title={PCR artifacts in LOH and MSI analysis of microdissected tumor cells.},
  author={Nathalie L G Sieben and Natalja Tatjana ter Haar and Cees J. Cornelisse and Gert Jan J Fleuren and Anne Marie Cleton-Jansen},
  journal={Human pathology},
  year={2000},
  volume={31 11},
  pages={1414-9}
}
Polymerase chain reaction (PCR) analysis to study loss of heterozygosity (LOH) and microsatellite instability (MSI) in tumors is widely used. Microdissection techniques are applied to obtain tumor-specific tissue cells. By microdissection, however, the amount of template DNA extracted may vary considerably and interfere with optimal PCR amplification. To circumvent LOH and MSI misinterpretations due to low DNA input, we have assessed the critical level of DNA input for reliable PCR analysis… CONTINUE READING

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