PCR and RFLP analyses based on the ribosomal protein operon.

@article{Martini2013PCRAR,
  title={PCR and RFLP analyses based on the ribosomal protein operon.},
  author={Marta Martini and Ing-Ming Lee},
  journal={Methods in molecular biology},
  year={2013},
  volume={938},
  pages={173-88}
}
Differentiation and classification of phytoplasmas have been primarily based on the highly conserved 16S rRNA gene. RFLP analysis of 16S rRNA gene sequences has identified 31 16S rRNA (16Sr) groups and more than 100 16Sr subgroups. Classification of phytoplasma strains can, however, become more refined and specific if moderately conserved genes, such as the… CONTINUE READING