PCR and PCR-RFLP techniques targeting the DNA topoisomerase II gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis.

@article{Kamiya2004PCRAP,
  title={PCR and PCR-RFLP techniques targeting the DNA topoisomerase II gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis.},
  author={Atsushi Kamiya and Akihiko Kikuchi and Yasushi Tomita and Toshio Kanbe},
  journal={Journal of dermatological science},
  year={2004},
  volume={34 1},
  pages={35-48}
}
BACKGROUND We have focused on the DNA topoisomerase II genes of several pathogenic fungi, and developed polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) methods targeting this gene for identification of dermatophytes. OBJECTIVE To assess the availability of the PCR-based identification for an etiologic study of dermatophytosis, by testing these PCR and PCR-RFLP methods for stability and reproducibility. METHODS Three hundred and fifty-six dermatophyte… CONTINUE READING
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