PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5′ -anchored simple sequence repeat (SSR) primers

@article{Charters1996PCRAO,
  title={PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5′ -anchored simple sequence repeat (SSR) primers},
  author={Yvonne Charters and Ashley B. Robertson and Michael James Wilkinson and Gavin Ramsay},
  journal={Theoretical and Applied Genetics},
  year={1996},
  volume={92},
  pages={442-447}
}
Primers complementary to simple sequence repeats (SSRs) and with variable three-base ‘anchors’ at their 5′ end, were used in PCR analyses to compare pooled DNA samples from various Brassica napus and B. rapa cultivars. Amplification products were resolved on polyacrylamide gels and detected by silver-nitrate staining. The resulting banding patterns were highly repeatable between replicate PCRs. Two of the primers produced polymorphisms at 33 and 23 band positions, respectively, and could each… CONTINUE READING

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