PCR amplification of species-specific DNA sequences can distinguish among Phytophthora species.

  title={PCR amplification of species-specific DNA sequences can distinguish among Phytophthora species.},
  author={Tibor {\'E}rsek and James E Schoelz and James T English},
  journal={Applied and environmental microbiology},
  volume={60 7},
We used PCR to differentiate species in the genus Phytophthora, which contains a group of devastating plant pathogenic fungi. We focused on Phytophthora parasitica, a species that can infect solanaceous plants such as tomato, and on Phytophthora citrophthora, which is primarily a citrus pathogen. Oligonucleotide primers were derived from sequences of a 1,300-bp P. parasitica-specific DNA segment and of an 800-bp P. citrophthora-specific segment. Under optimal conditions, the primers developed… CONTINUE READING

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