PCR amplification of repetitive DNA: a limitation to genome editing technologies and many other applications

@inproceedings{Hommelsheim2014PCRAO,
  title={PCR amplification of repetitive DNA: a limitation to genome editing technologies and many other applications},
  author={Carl Maximilian Hommelsheim and Lamprinos Frantzeskakis and Mengmeng Huang and B. Ayça {\"U}lker},
  booktitle={Scientific reports},
  year={2014}
}
Designer transcription-activator like effectors (TALEs) is a promising technology and made it possible to edit genomes with higher specificity. Such specific engineering and gene regulation technologies are also being developed using RNA-binding proteins like PUFs and PPRs. The main feature of TALEs, PUFs and PPRs is their repetitive DNA/RNA-binding domains which have single nucleotide binding specificity. Available kits today allow researchers to assemble these repetitive domains in any… CONTINUE READING
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