PCR amplification of murine immunoglobulin germline V genes: strategies for minimization of recombination artefacts.

@article{Zylstra1998PCRAO,
  title={PCR amplification of murine immunoglobulin germline V genes: strategies for minimization of recombination artefacts.},
  author={Paula Zylstra and Harald S. Rothenfluh and George F Weiller and Robert V. Blanden and E J Steele},
  journal={Immunology and cell biology},
  year={1998},
  volume={76 5},
  pages={
          395-405
        }
}
Murine immunoglobulin germline V genes exist as multiple sequences arranged in tandem in germline DNA. Because members of V gene families are very similar, they can be amplified simultaneously using the polymerase chain reaction (PCR) with a single set of primers designed over regions of sequence similarity. In the present paper, the variables relevant to production of artefacts by recombination between different germline sequences during amplification are investigated. Pfu or Taq DNA… CONTINUE READING

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