PCR-amplification of GC-rich regions: 'slowdown PCR'

@article{Frey2008PCRamplificationOG,
  title={PCR-amplification of GC-rich regions: 'slowdown PCR'},
  author={Ulrich H. Frey and Hagen Sjard Bachmann and J{\"u}rgen Peters and Winfried Siffert},
  journal={Nature Protocols},
  year={2008},
  volume={3},
  pages={1312-1317}
}
The polymerase chain reaction (PCR) technique has become an indispensable method in molecular research. [] Key Method The protocol relies on the addition of 7-deaza-2′-deoxyguanosine, a dGTP analog to the PCR mixture and a novel standardized cycling protocol with varying temperatures. The latter consists of a generally lowered ramp rate of 2.5 °C s−1 and a low cooling rate of 1.5 °C s−1 for reaching an annealing temperature and is run for 48 cycles. We established this protocol as a versatile method not only…

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