PCR amplification of DNA containing non-standard base pairs by variants of reverse transcriptase from Human Immunodeficiency Virus-1.

@article{Sismour2004PCRAO,
  title={PCR amplification of DNA containing non-standard base pairs by variants of reverse transcriptase from Human Immunodeficiency Virus-1.},
  author={A. Michael Sismour and Stefan Lutz and Chong-Ook Park and Michael J. Lutz and Paul L. Boyer and Stephen H. Hughes and Steven A. Benner},
  journal={Nucleic acids research},
  year={2004},
  volume={32 2},
  pages={728-35}
}
As the next step towards generating a synthetic biology from artificial genetic information systems, we have examined variants of HIV reverse transcriptase (RT) for their ability to synthesize duplex DNA incorporating the non-standard base pair between 2,4-diaminopyrimidine (pyDAD), a pyrimidine presenting a hydrogen bond 'donor-acceptor-donor' pattern to the complementary base, and xanthine (puADA), a purine presenting a hydrogen bond 'acceptor-donor-acceptor' pattern. This base pair fits the… CONTINUE READING

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