PCR amplification and polymorphism analysis of the intergenic spacer region of ribosomal DNA in Tuber borchii.

@article{Ciarmela2002PCRAA,
  title={PCR amplification and polymorphism analysis of the intergenic spacer region of ribosomal DNA in Tuber borchii.},
  author={Pasquapina Ciarmela and Lucia Potenza and Luigi Cucchiarini and Sabrina Zeppa and Vilberto Stocchi},
  journal={Microbiological research},
  year={2002},
  volume={157 1},
  pages={69-74}
}
PCR amplification of the complete intergenic spacer region (IGS) of the Tuber borchii nuclear ribosomal repeat was obtained using universal primers CNL 12 and NS1rev. In order to improve amplification yield a specific primer, T1, was selected from a partial sequence of the IGS product. IGS diversity was characterized both at the intraindividual and intraspecific level. The results obtained at the intraindividual level showed 10% varying repeats on ten screened colonies, while at the… CONTINUE READING

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