• Biology
  • Published 2006

PCR-RFLP and bioinformatic assay of urease B gene from different helicobacter pylori strains

@inproceedings{Quanming2006PCRRFLPAB,
  title={PCR-RFLP and bioinformatic assay of urease B gene from different helicobacter pylori strains},
  author={Zou Quan-ming},
  year={2006}
}
Objective:To analyze the polymorphism of urease B gene(ureB) from different Hp strains. Methods:The sequences of ureB gene from 14 different Hp strains were amplified by PCR to analyze PCR-RFLP with HaeⅢ digestion.At the same time,the sequence of ureB gene was analyzed with biochemical software to compare the homology of nucleotide and amino acid sequence and to profile the phylogenetic tree. Results: The results of 1.7 kb ureB gene digested with HaeⅢ showed there were 2-5 band types in 14… CONTINUE READING