PCR-RFLP analysis for identification of Tetranychus spider mite species (Acari: Tetranychidae).

@article{Arimoto2013PCRRFLPAF,
  title={PCR-RFLP analysis for identification of Tetranychus spider mite species (Acari: Tetranychidae).},
  author={Makoto Arimoto and Masaru Satoh and Ryuji Uesugi and Masahiro M H Osakabe},
  journal={Journal of economic entomology},
  year={2013},
  volume={106 2},
  pages={661-8}
}
A polymerase chain reaction (PCR)-restriction fragment length polymorphism (PCR-restriction fragment-length polymorphism)-based method for species identification was applied to 14 Tetranychus spider mite species, which were dominant species intercepted at Japanese import plant quarantine. We sequenced the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (rDNA), which included the partial ends of the 18S and 28S ribosomal RNA genes, 5.8S ribosomal RNA gene, and two internal… CONTINUE READING