PCR Method of Detecting Pork in Foods for Verifying Allergen Labeling and for Identifying Hidden Pork Ingredients in Processed Foods

@article{Tanabe2007PCRMO,
  title={PCR Method of Detecting Pork in Foods for Verifying Allergen Labeling and for Identifying Hidden Pork Ingredients in Processed Foods},
  author={Soichi Tanabe and Eiji Miyauchi and Akemi Muneshige and Kazuhiro Mio and Chikara Sato and Masahiko Sato},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2007},
  volume={71},
  pages={1663 - 1667}
}
A PCR method to detect porcine DNA was developed for verifying the allergen labeling of foods and for identifying hidden pork ingredients in processed foods. The primer pair, F2/R1, was designed to detect the gene encoding porcine cytochrome b for the specific detection of pork with high sensitivity. The amplified DNA fragment (130 bp) was specifically detected from porcine DNA, while no amplification occurred with other species such as cattle, chicken, sheep, and horse. When the developed PCR… 
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