PCR Incorporation of Polyoxometalate Modified Deoxynucleotide Triphosphates and Their Application in Molecular Electrochemical Sensing of Yersinia pestis.

@article{Ortiz2017PCRIO,
  title={PCR Incorporation of Polyoxometalate Modified Deoxynucleotide Triphosphates and Their Application in Molecular Electrochemical Sensing of Yersinia pestis.},
  author={Mayreli Ortiz and Ahmed M Debela and Marketa Svobodova and Serge Thorimbert and Denis Lesage and Richard B Cole and Bernold Hasenknopf and Ciara K O'Sullivan},
  journal={Chemistry},
  year={2017},
  volume={23 44},
  pages={10597-10603}
}
Redox-labeled nucleotides are of increasing interest for the fabrication of next generation molecular tools and should meet requirements of being thermally stable, sensitive, and compatible with polymerase-mediated incorporation while also being electrochemically discriminable. The synthesis and characterization of Keggin and Dawson polyoxometalate-deoxynucleotide (POM-dNTP) bioconjugates linked through 7-deaza-modified purines is described. The modified POM-dNTPs were used for polymerase-based… CONTINUE READING