PCR-Enzyme immunoassay for detection of Streptococcus pneumoniae DNA in cerebrospinal fluid samples from patients with culture-negative meningitis.

@article{Cherian1998PCREnzymeIF,
  title={PCR-Enzyme immunoassay for detection of Streptococcus pneumoniae DNA in cerebrospinal fluid samples from patients with culture-negative meningitis.},
  author={Thomas Cherian and Mukkai Kesavan Lalitha and A. C. Manoharan and Kurien Thomas and Robert Yolken and Mark C. Steinhoff},
  journal={Journal of clinical microbiology},
  year={1998},
  volume={36 12},
  pages={3605-8}
}
A PCR-based assay was developed to amplify a conserved region of the pneumococcal autolysin gene. The amplified product was labelled with digoxigenin-labelled dUTP and was detected with a biotin-labelled probe in an enzyme immunoassay (EIA). The assay was initially tested with suspensions of various serotypes of Streptococcus pneumoniae and other gram-positive and gram-negative bacteria and was then applied to cerebrospinal fluid (CSF) specimens from patients with meningitis and those with… CONTINUE READING
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