PCR-Based methods for identification ofEnterococcus species

@article{Drahovsk2002PCRBasedMF,
  title={PCR-Based methods for identification ofEnterococcus species},
  author={Hana Drahovsk{\'a} and Dana Koc{\'i}ncov{\'a} and Milan Seman and Jan Turna},
  journal={Folia Microbiologica},
  year={2002},
  volume={47},
  pages={649-653}
}
Two DNA-based techniques were used for species identification of enterococci.PvuII digestion of the genus-specific PCR product yielded four different restriction profiles among 20 enterococcal species; one of them was species-specific forE. faecium. In the second case, 32 reference strains belonging to 20 enterococcal species were divided to 12 groups by amplification of internal transcribed spacer of rRNA operon. Interspecies and some intraspecies profile variability was determined. Both… CONTINUE READING

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