PCR Analysis of cry7 Genes in Bacillus thuringiensis by the Five Conserved Blocks of Toxins

@article{BenDov2001PCRAO,
  title={PCR Analysis of cry7 Genes in Bacillus thuringiensis by the Five Conserved Blocks of Toxins},
  author={Eitan Ben-Dov and Robert Manasherob and Arieh Zaritsky and Ze'ev Barak and Yoel Margalith},
  journal={Current Microbiology},
  year={2001},
  volume={42},
  pages={96-99}
}
An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes. 

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