PCR ASSAY DETECTS MANNHEIMIA HAEMOLYTICA IN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010

@inproceedings{Shanthalingam2014PCRAD,
  title={PCR ASSAY DETECTS MANNHEIMIA HAEMOLYTICA IN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010},
  author={Sudarvili Shanthalingam and Andrea Goldy and Jegarubee Bavananthasivam and Renuka Subramaniam and Sai Arun Batra and Abirami Kugadas and Bindu Raghavan and Rohana P. Dassanayake and Jessica E. Jennings-Gaines and Halcyon J. Killion and William H. Edwards and Jennifer M Ramsey and Neil J. Anderson and Peregrine L. Wolff and Kristin G. Mansfield and Darren L. Bruning and Subramaniam Srikumaran},
  booktitle={Journal of wildlife diseases},
  year={2014}
}
Abstract Mannheimia haemolytica consistently causes severe bronchopneumonia and rapid death of bighorn sheep (Ovis canadensis) under experimental conditions. However, Bibersteinia trehalosi and Pasteurella multocida have been isolated from pneumonic bighorn lung tissues more frequently than M. haemolytica by culture-based methods. We hypothesized that assays more sensitive than culture would detect M. haemolytica in pneumonic lung tissues more accurately. Therefore, our first objective was to… Expand
Rapid detection of Mannheimia haemolytica in lung tissues of sheep and from bacterial culture
TLDR
The present study emphasized the PCR as a valuable tool for rapid detection of M. haemolytica in clinical cases of pneumonia and other disease manifestations in sheep and other ruminants, thereby providing the basis for effective preventive strategies. Expand
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TLDR
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TLDR
This study is the first to apply state of the art molecular methods to shed light on the nasopharyngeal carrier state of Pasteurellaceae in camels, demonstrating a hitherto unrecognised active carrier state for M. granulomatis or a closely related new Mannheimia species from camelids. Expand
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TLDR
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AN ECOLOGIC STUDY COMPARING DISTRIBUTION OF PASTEURELLA TREHALOSI AND MANNHEIMIA HAEMOLYTICA BETWEEN SIERRA NEVADA BIGHORN SHEEP, WHITE MOUNTAIN BIGHORN SHEEP, AND DOMESTIC SHEEP
TLDR
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