PCR ASSAY DETECTS MANNHEIMIA HAEMOLYTICA IN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010

@inproceedings{Shanthalingam2014PCRAD,
  title={PCR ASSAY DETECTS MANNHEIMIA HAEMOLYTICA IN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010},
  author={Sudarvili Shanthalingam and Andrea Goldy and Jegarubee Bavananthasivam and Renuka Subramaniam and Sai Arun Batra and Abirami Kugadas and Bindu Raghavan and Rohana P. Dassanayake and Jessica E Jennings-Gaines and Halcyon J. Killion and William H. Edwards and Jennifer M. Ramsey and Neil J. Anderson and Peregrine L. Wolff and Kristin G. Mansfield and Darren L. Bruning and Subramaniam Srikumaran},
  booktitle={Journal of wildlife diseases},
  year={2014}
}
Abstract Mannheimia haemolytica consistently causes severe bronchopneumonia and rapid death of bighorn sheep (Ovis canadensis) under experimental conditions. However, Bibersteinia trehalosi and Pasteurella multocida have been isolated from pneumonic bighorn lung tissues more frequently than M. haemolytica by culture-based methods. We hypothesized that assays more sensitive than culture would detect M. haemolytica in pneumonic lung tissues more accurately. Therefore, our first objective was to… 
Rapid detection of Mannheimia haemolytica in lung tissues of sheep and from bacterial culture
TLDR
The present study emphasized the PCR as a valuable tool for rapid detection of M. haemolytica in clinical cases of pneumonia and other disease manifestations in sheep and other ruminants, thereby providing the basis for effective preventive strategies.
Fusobacterium necrophorum in North American Bighorn Sheep (Ovis canadensis) Pneumonia
TLDR
Fusobacterium leukotoxin is highly toxic for BHS leukocytes; however, based on the PCR findings, it is unlikely to play a direct role in the development of BHS pneumonia.
Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocida associated with pneumonia of goats in Chhattisgarh
TLDR
The present study reported an occurrence of pneumonic pasteurellosis caused by M. haemolytica and P. multocida in a goat flock.
Growth of Mannheimia haemolytica: inhibitory agents and putative mechanism of inhibition.
Immunohistochemical and Molecular Detection of Mannheimia spp . and Pasteurella spp . in Sheep with Pneumonia in Kars Province-Turkey
TLDR
The results of the study showed that Mannheimia haemolytica is an important cause of pneumonia in sheep in Kars, and Pasteurella multocida, although with lesser importance can cause pneumonia in this species.
Biological and molecular characterization of a sheep pathogen isolate of Mannheimia haemolytica and leukotoxin production kinetics
TLDR
The biological and molecular characteristics of a new Moroccan Mha isolate from sheep that died of pneumonia was characterized for the 1st time in North Africa using biological and Molecular methods.
β-Hemolysis May Not Be a Reliable Indicator of Leukotoxicity of Mannheimia haemolytica Isolates
TLDR
Results suggest that β-hemolysis may not be a reliable indicator of leukotoxic and hemolytic properties of M. haemolytica isolates that possess lktA gene may not secrete activeLeukotoxin.
Epizootic Pneumonia of Bighorn Sheep following Experimental Exposure to Mycoplasma ovipneumoniae
TLDR
The epidemiologic, pathologic and microbiologic findings in these experimental animals resembled those seen in naturally occurring pneumonia outbreaks in free ranging bighorn sheep.
Association between Mannheimia haemolytica infection with reproductive physiology and performance in small ruminants: A review
TLDR
This review will explore the possibilities of involvement and new potential research to be carried out to determine the involvement of male and female reproductive system with M. haemolytica infection among small ruminants.
Molecular study on Pasteurella multocida and Mannheimia granulomatis from Kenyan Camels (Camelus dromedarius)
TLDR
This study is the first to apply state of the art molecular methods to shed light on the nasopharyngeal carrier state of Pasteurellaceae in camels, demonstrating a hitherto unrecognised active carrier state for M. granulomatis or a closely related new Mannheimia species from camelids.
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References

SHOWING 1-10 OF 26 REFERENCES
Use of DNA analysis of Pasteurella haemolytica biotype T isolates to monitor transmission in bighorn sheep (Ovis canadensis canadensis)
TLDR
Pneumonia has been identified as a major cause of poor lamb survival in indigenous herds of Rocky Mountain bighorn sheep in central Idaho and Pasteurella haemolytica biotype T isolates from the ewes and lambs, as well as those from a 9-month-old lamb, were subjected to HaeIII restriction enzyme analysis (REA) and ribotyping.
Association of Mycoplasma ovipneumoniae Infection with Population-Limiting Respiratory Disease in Free-Ranging Rocky Mountain Bighorn Sheep (Ovis canadensis canadensis)
TLDR
M. ovipneumoniae is strongly associated with bronchopneumonia in free-ranging bighorn sheep and is a candidate primary etiologic agent for this disease.
Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida
TLDR
Results indicate that P. multocida inhibits the growth of M. haemolytica from the lungs of pneumonic BHS by a contact- or proximity-dependent mechanism.
AN ECOLOGIC STUDY COMPARING DISTRIBUTION OF PASTEURELLA TREHALOSI AND MANNHEIMIA HAEMOLYTICA BETWEEN SIERRA NEVADA BIGHORN SHEEP, WHITE MOUNTAIN BIGHORN SHEEP, AND DOMESTIC SHEEP
TLDR
No clear evidence of association was found in the Pasteurellaceae distribution between the White Mountains bighorn sheep and domestic sheep, although these comparisons had relatively large confidence intervals for the point estimates.
Bibersteinia trehalosi Inhibits the Growth of Mannheimia haemolytica by a Proximity-Dependent Mechanism
TLDR
Growth curves (monoculture) demonstrated that B. trehalosi can both outgrow and inhibit M. haemolytica growth with the latter related to a proximity- or contact-dependent mechanism.
Detection of Mycoplasma ovipneumoniae and M. arginini in Bighorn Sheep Using Enrichment Culture Coupled with Genus- and Species-Specific Polymerase Chain Reaction
TLDR
A culture protocol that employed an average initial 3-day enrichment culture in liquid Hayflick broth in a CO2-enhanced atmosphere and two methods of transport from field to laboratory gave similar results under study conditions.
A Bighorn Sheep Die-off in Southern Colorado Involving a Pasteurellaceae Strain that May Have Originated from Syntopic Cattle
TLDR
Field and laboratory observations suggested that pneumonia in affected bighorns may have been caused by a combination of pathogens including two pathogenic Pasteurellaceae strains—one likely of cattle origin and one likely of bighorn origin—with infections in some cases perhaps exacerbated by other respiratory pathogens and severe weather conditions.
Role of Bibersteinia trehalosi, respiratory syncytial virus, and parainfluenza-3 virus in bighorn sheep pneumonia.
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