PCNA, the Maestro of the Replication Fork

@article{Moldovan2007PCNATM,
  title={PCNA, the Maestro of the Replication Fork},
  author={George-Lucian Moldovan and B. Pfander and S. Jentsch},
  journal={Cell},
  year={2007},
  volume={129},
  pages={665-679}
}
Inheritance requires genome duplication, reproduction of chromatin and its epigenetic information, mechanisms to ensure genome integrity, and faithful transmission of the information to progeny. Proliferating cell nuclear antigen (PCNA)-a cofactor of DNA polymerases that encircles DNA-orchestrates several of these functions by recruiting crucial players to the replication fork. Remarkably, many factors that are involved in replication-linked processes interact with a particular face of PCNA and… Expand
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References

SHOWING 1-10 OF 165 REFERENCES
Interaction with PCNA is essential for yeast DNA polymerase eta function.
TLDR
Evidence is presented for the physical and functional interaction of yeast Poleta with proliferating cell nuclear antigen (PCNA) and it is shown that the interaction with PCNA is essential for the in vivo function of PoletA. Expand
PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA
TLDR
This work has shown that DNA cytosine methyltransferase 1 (Dnmt1), which preserves epigenetic signals by completing the methylation of hemimethylated DNA after DNA replication, is one of these PCNA binding proteins that interacts with a number of other proteins to increase their local concentration at replicated DNA sites. Expand
SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase
TLDR
It is shown by genetic analysis that SUMO-modified PCNA functionally cooperates with Srs2, a helicase that blocks recombinational repair by disrupting Rad51 nucleoprotein filaments, which suggests a model in whichsumO- modified PCNA recruits SRS2 in S phase in order to prevent unwanted recombination events of replicating chromosomes. Expand
A Role for PCNA Ubiquitination in Immunoglobulin Hypermutation
TLDR
This is the first evidence, to the authors' knowledge, that vertebrates exploit the PCNA-ubiquitin pathway for immunoglobulin hypermutation, most likely through the recruitment of error-prone DNA polymerases. Expand
PCNA connects DNA replication to epigenetic inheritance in yeast
Formation of a heterochromatin-like structure results in transcriptional silencing at the HM mating-type loci and telomeres in Saccharomyces cerevisiae. Once formed, such epigenetically determinedExpand
PCNA functions as a molecular platform to trigger Cdt1 destruction and prevent re-replication
TLDR
It is reported that replication-dependent proteolysis of Cdt1 requires its interaction with proliferating cell nuclear antigen (PCNA), a homotrimeric processivity factor for DNA polymerases to be inactivated. Expand
The PCNA-RFC families of DNA clamps and clamp loaders.
  • J. Majka, P. Burgers
  • Biology, Medicine
  • Progress in nucleic acid research and molecular biology
  • 2004
TLDR
Multiple stepwise ATP-binding events to RFC are required to load PCNA onto primed DNA, and this stepwise mechanism should permit editing of this process at individual steps and allow for divergence of the default process into more specialized modes. Expand
Interaction between PCNA and Diubiquitinated Mcm10 Is Essential for Cell Growth in Budding Yeast
TLDR
Interestingly, a Y245A substitution in the PIP box of Mcm10 that inhibits the interaction with PCNA abolishes cell proliferation, which has not been observed for analogous mutations in other PCNA-interacting proteins. Expand
Ubiquitin-Binding Domains in Y-Family Polymerases Regulate Translesion Synthesis
TLDR
The identification of two ubiquitin (Ub)–binding domains (UBM and UBZ), which are evolutionarily conserved in all Y-family TLS polymerases (pols), indicate that Ub-binding domains of Y- family polymerases play crucial regulatory roles in TLS. Expand
Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases eta and REV1.
  • P. Garg, P. Burgers
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2005
TLDR
A robust in vitro system that ubiquitination of PCNA increases its functionality as a sliding clamp to promote mutagenic DNA replication and activates two key enzymes in translesion synthesis. Expand
...
1
2
3
4
5
...