PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: in vitro cell culture studies.

Abstract

In the present study, we examined the potential of using highly porous poly(ε-caprolactone) (PCL)-coated hydroxyapatite (HAp) scaffold derived from cuttlefish bone for bone tissue engineering applications. The cell culture studies were performed in vitro with preosteoblastic MC3T3-E1 cells in static culture conditions. Comparisons were made with uncoated HAp scaffold. The attachment and spreading of preosteoblasts on scaffolds were observed by Live/Dead staining Kit. The cells grown on the HAp/PCL composite scaffold exhibited greater spreading than cells grown on the HAp scaffold. DNA quantification and scanning electron microscopy (SEM) confirmed a good proliferation of cells on the scaffolds. DNA content on the HAp/PCL scaffold was significantly higher compared to porous HAp scaffolds. The amount of collagen synthesis was determined using a hydroxyproline assay. The osteoblastic differentiation of the cells was evaluated by determining alkaline phosphatase (ALP) activity and collagen type I secretion. Furthermore, cell spreading and cell proliferation within scaffolds were observed using a fluorescence microscope.

DOI: 10.1016/j.msec.2014.05.034
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@article{Milovac2014PCLcoatedHS, title={PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: in vitro cell culture studies.}, author={Dajana Milovac and Tatiana C Gamboa-Mart{\'i}nez and Marica Ivankovi{\'c} and Gloria Gallego Ferrer and Hrvoje Ivankovi{\'c}}, journal={Materials science & engineering. C, Materials for biological applications}, year={2014}, volume={42}, pages={264-72} }