PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea

@article{Agarwal2006PCIBAA,
  title={PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea},
  author={Pradeep Kumar Agarwal and Parinita Agarwal and Jan B. M. Custers and Chun-ming Liu and Sant Saran Bhojwani},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2006},
  volume={86},
  pages={201-210}
}
An efficient protocol to improve microspore embryogenesis is established in an important oleiferous crop, Brassica juncea (Indian mustard). Colchicine was used for enhancing microspore embryogenesis and also to obtain doubled haploid embryos. Colchicine at high concentrations (>10 mg l−1), for 24 h, proved convenient for direct recovery of diploid embryos. Higher temperature treatment and an antiauxin PCIB (p-chlorophenoxyisobutyric acid) enhanced microspore embryogenesis significantly as… CONTINUE READING
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