PAT-seq: a method to study the integration of 3'-UTR dynamics with gene expression in the eukaryotic transcriptome.

@article{Harrison2015PATseqAM,
  title={PAT-seq: a method to study the integration of 3'-UTR dynamics with gene expression in the eukaryotic transcriptome.},
  author={Paul F Harrison and David R Powell and Jennifer L. Clancy and Thomas Preiss and Peter R Boag and Ana Traven and Torsten Seemann and Traude H Beilharz},
  journal={RNA},
  year={2015},
  volume={21 8},
  pages={
          1502-10
        }
}
A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, we developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. PAT-seq returns (i) digital gene expression, (ii) polyadenylation site/s, and (iii) the polyadenylation-state within and between eukaryotic transcriptomes. PAT-seq differs from previous 3' focused RNA… CONTINUE READING

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