PAGE analysis of the heteroduplexes formed between PCR-amplified 16S rRNA genes: estimation of sequence similarity and rDNA complexity.

@article{Espejo1998PAGEAO,
  title={PAGE analysis of the heteroduplexes formed between PCR-amplified 16S rRNA genes: estimation of sequence similarity and rDNA complexity.},
  author={Romilio T. Espejo and Carmen G Feij{\'o}o and Jaime Romero and M{\^o}nica Lopez Vasquez},
  journal={Microbiology},
  year={1998},
  volume={144 ( Pt 6)},
  pages={1611-7}
}
Analysis of the 16S rRNA genes retrieved directly from different environments has proven to be a powerful tool that has greatly expanded our knowledge of microbial diversity and phylogeny. It is shown here that sequence similarity between 80 and 100% among 16S rDNAs can be estimated by the electrophoretic migration of their heteroduplexes. This was measured by hybridization and electrophoresis in polyacrylamide gels of the product obtained after PCR amplification of almost the entire 16S rRNA… CONTINUE READING

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