P228: Follow-up of patient colonization with multidrug-resistant microorganisms in the intensive care unit and after discharge

Abstract

Methods It is a prospective cohort with a follow-up of patients colonized with multidrug-resistant microorganisms during the length of stay and after discharge at two adult general ICUs from two different hospitals in Belo Horizonte, Brazil. All patients colonized or infected with resistant microorganisms, from April 2012 to January 2013, were monitored with microbiological cultures every 15 days during their length of stay at the ICU and every month after discharge until natural decolonization. Specimens were collected with a sterile swab pre-moistened with sterile saline solution. Screening were conducted by sampling the anterior nares, groin, perineum and wounds. Samples were plated on MacConkey, Cetrimide, Baird Parker agar and, Bile Esculin agar and incubated for 48 h at 37oC. Bacteria isolated were submitted for Gram stain, catalase and Vitek (BioMérieux) tests and, for antibiogram by Bauer-Kirby method (imipenem, oxacillin, cefoxitin and ceftriaxone). For Staphylococcus spp the minimal inhibition concentration (MIC) of vancomycin were determined.

DOI: 10.1186/2047-2994-2-S1-P228

Cite this paper

@inproceedings{Damaceno2013P228FO, title={P228: Follow-up of patient colonization with multidrug-resistant microorganisms in the intensive care unit and after discharge}, author={QS Damaceno and A A Oliveira and J Nicoli}, booktitle={Antimicrobial Resistance and Infection Control}, year={2013} }