P-glycoprotein Expression, Localization, and Function in Sandwich-Cultured Primary Rat and Human Hepatocytes: Relevance to the

@inproceedings{Hoffmaster2004PglycoproteinEL,
  title={P-glycoprotein Expression, Localization, and Function in Sandwich-Cultured Primary Rat and Human Hepatocytes: Relevance to the},
  author={Keith Hoffmaster and Ryan Z. Turncliff and Edward L. Lecluyse and Richard B Kim and Peter J. Meier and Kim L R Brouwer},
  year={2004}
}
Purpose. The isolation of hepatocytes from intact liver involves collagenase digestion of the tissue, resulting in loss of cell polarization and functional vectorial excretion. These studies examined repolarization, localization of P-glycoprotein (P-gp) to the canalicular domain of the hepatocyte, and re-establishment of vectorial transport in sandwich-cultured (SC) rat and human primary hepatocytes. Methods. Protein localization and expression were determined in SC hepatocytes by confocal… CONTINUE READING