P-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic drosophila melanogaster

@article{Zehring1984PelementTW,
  title={P-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic drosophila melanogaster},
  author={William A. Zehring and David A. Wheeler and Pranhitha Reddy and Ronald J. Konopka and Charalambos P. Kyriacou and Michael Rosbash and Jeffrey C. Hall},
  journal={Cell},
  year={1984},
  volume={39},
  pages={369-376}
}
Mutations at the period (per) locus of Drosophila melanogaster disrupt several biological rhythms. Molecular cloning of DNA sequences encompassing the per+ locus has allowed germ-line transformation experiments to be carried out. Certain subsegments of the per region, transduced into the genome of arrhythmic pero flies, restore rhythmicity in circadian locomotor behavior and the male's courtship song. 
Germ-line transformation involving DNA from the period locus in Drosophila melanogaster: overlapping genomic fragments that restore circadian and ultradian rhythmicity to per0 and per- mutants.
P-element-mediated transformations involving DNA fragments from the period (per) clock gene of Drosophila melanogaster have shown that several subsegments of the locus restore rhythmicity to per0 orExpand
Behaviour modification by in vitro mutagenesis of a variable region within the period gene of Drosophila
TLDR
The phenotypes of transformed fruit flies, in which the only functional period gene lacks the entire perfect threonine-glycine repeat region, show that the effects of the period gene on the circadian and male courtship song rhythms can be dissociated. Expand
Dosage compensation of the period gene in Drosophila melanogaster.
TLDR
The results suggest that per contains multipartite regulatory information for dosage compensation within the large first intron and also within the 3' half of this genetic locus. Expand
A promoterless period gene mediates behavioral rhythmicity and cyclical per expression in a restricted subset of the drosophila nervous system
TLDR
Cycling of PER in brain cells of flies carrying the same 7.2 kb piece of per DNA under the control of a heat shock promoter corroborated the hypothesis that per's molecular cyclings and behavioral rhythmicity are causally related. Expand
Changes in abundance or structure of the per gene product can alter periodicity of the Drosophila clock
TLDR
It is suggested that perl and pers mutants produce hypoactive and hyperactive per proteins, respectively, which are inversely correlated with period length, so that flies with lowest levels of the per product have slow-running biological clocks. Expand
An inducible promoter fused to the period gene in Drosophila conditionally rescues adult per-mutant arrhythmicity
TLDR
Experiments with arrhythmic per mutants bearing an inducible form of this gene indicate that strongly rhythmic adult behaviour can be obtained only if per expression is induced in the adult, independent of its history of expression earlier in development. Expand
Product of per locus of Drosophila shares homology with proteoglycans
TLDR
A fragment of DNA of ∼7 kilobases (kb) encoding a 4.5-kb poly(A)+ RNA restores rhyth-micity when transduced into Drosophila carrying mutations5,6 or chromosomal deletions5 of the per locus and the sequence of this biologically active segment of DNA is reported. Expand
Requirement for period gene expression in the adult and not during development for locomotor activity rhythms of imaginal Drosophila melanogaster.
TLDR
Evidence is presented suggesting that it is necessary for pacemaker function itself, rather than being involved in a process that couples the activity of the pacemaker to the output pathway, and can rescue the host's arrhythmic phenotype even when supplied many days after transfer to constant darkness. Expand
Functional Identification of the Mouse Circadian Clock Gene by Transgenic BAC Rescue
TLDR
In vivo complementation with bacterial artificial chromosome clones expressed in transgenic mice to identify the circadian Clock gene provides a proof of principle that "cloning by rescue" is an efficient and definitive method in mice. Expand
An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates
TLDR
The results of a search for sequences homologous to the per locus DNA in the genomic DNA of several species of vertebrates show an unusual, tandemly repeated sequence forming a portion of the 4.5-kb per transcript is homologously to DNA in chicken, mouse and man. Expand
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