P-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic drosophila melanogaster

@article{Zehring1984PelementTW,
  title={P-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic drosophila melanogaster},
  author={William A. Zehring and David A. Wheeler and Pranhitha Reddy and Ronald J. Konopka and Charalambos P. Kyriacou and Michael Rosbash and Jeffrey C. Hall},
  journal={Cell},
  year={1984},
  volume={39},
  pages={369-376}
}
Germ-line transformation involving DNA from the period locus in Drosophila melanogaster: overlapping genomic fragments that restore circadian and ultradian rhythmicity to per0 and per- mutants.
P-element-mediated transformations involving DNA fragments from the period (per) clock gene of Drosophila melanogaster have shown that several subsegments of the locus restore rhythmicity to per0 or
Behaviour modification by in vitro mutagenesis of a variable region within the period gene of Drosophila
TLDR
The phenotypes of transformed fruit flies, in which the only functional period gene lacks the entire perfect threonine-glycine repeat region, show that the effects of the period gene on the circadian and male courtship song rhythms can be dissociated.
Dosage compensation of the period gene in Drosophila melanogaster.
TLDR
The results suggest that per contains multipartite regulatory information for dosage compensation within the large first intron and also within the 3' half of this genetic locus.
Changes in abundance or structure of the per gene product can alter periodicity of the Drosophila clock
TLDR
It is suggested that perl and pers mutants produce hypoactive and hyperactive per proteins, respectively, which are inversely correlated with period length, so that flies with lowest levels of the per product have slow-running biological clocks.
An inducible promoter fused to the period gene in Drosophila conditionally rescues adult per-mutant arrhythmicity
TLDR
Experiments with arrhythmic per mutants bearing an inducible form of this gene indicate that strongly rhythmic adult behaviour can be obtained only if per expression is induced in the adult, independent of its history of expression earlier in development.
Product of per locus of Drosophila shares homology with proteoglycans
TLDR
A fragment of DNA of ∼7 kilobases (kb) encoding a 4.5-kb poly(A)+ RNA restores rhyth-micity when transduced into Drosophila carrying mutations5,6 or chromosomal deletions5 of the per locus and the sequence of this biologically active segment of DNA is reported.
Requirement for period gene expression in the adult and not during development for locomotor activity rhythms of imaginal Drosophila melanogaster.
TLDR
Evidence is presented suggesting that it is necessary for pacemaker function itself, rather than being involved in a process that couples the activity of the pacemaker to the output pathway, and can rescue the host's arrhythmic phenotype even when supplied many days after transfer to constant darkness.
An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates
TLDR
The results of a search for sequences homologous to the per locus DNA in the genomic DNA of several species of vertebrates show an unusual, tandemly repeated sequence forming a portion of the 4.5-kb per transcript is homologously to DNA in chicken, mouse and man.
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Physical characterization of a series of chromosomal rearrangements altering per locus activity indicates that DNA affecting behavioral rhythms is found in a 7.1-kb HindIII fragment.
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A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene.
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Three mutants have been isolated in which the normal 24-hour rhythm is drastically changed and all these mutations appear to involve the same functional gene on the X chromosome.
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TLDR
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TLDR
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TLDR
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