Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA.

@article{Sawadaishi1985OzonolysisOS,
  title={Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA.},
  author={K Sawadaishi and K. T. Miura and Eiko Ohtsuka and Tadashi Ueda and Kanji Ishizaki and Nariko Shinriki},
  journal={Nucleic acids research},
  year={1985},
  volume={13 20},
  pages={7183-94}
}
Treatment of supercoiled pBR322 DNA with ozone resulted in the conversion of closed circular DNA to open circular DNA. Restriction analysis of the resulting open circular DNA showed that ozonolysis in the absence of salt caused single strand cleavage at specific sites.