Oxytocin is hydrolyzed by an enzyme in human placenta that is identical to the oxytocinase of pregnancy serum

  title={Oxytocin is hydrolyzed by an enzyme in human placenta that is identical to the oxytocinase of pregnancy serum},
  author={Mieko Naruki and Shigehiko Mizutani and Keiko Goto and Masafumi Tsujimoto and Hitomi Nakazato and Atsuo Itakura and Kimio Mizuno and Osamu Kurauchi and Fumitaka Kikkawa and Yutaka Tomoda},

Immunoaffinity purification and characterization of native placental leucine aminopeptidase/oxytocinase from human placenta.

cDNA cloning of placental leucine aminopeptidase (P-LAP)/cystinyl aminopeptidase (CAP)/oxytocinase demonstrated that this enzyme is a type II integral membrane protein, which means that native P-LAP,

Placental Leucine Aminopeptidase- and Aminopeptidase A- Deficient Mice Offer Insight concerning the Mechanisms Underlying Preterm Labor and Preeclampsia

It is shown that APA acts as an antihypertensive agent in the pregnant spontaneously hypertensive rat by degrading vasoactive peptides and as a result returns the animal to a normotensive state, and P-LAP also acts as a antiuterotonic agent by degrading uterotonic peptide and thus prolongs gestation in the unborn mouse.

Physiological and pathophysiological roles of placental aminopeptidase in maternal sera: possible relation to preeclampsia and preterm delivery.

The emerging role of aminopeptidases in the monitoring and development of improved therapeutic strategies that provide better patient selection for therapeutic personalization is reviewed.

New insights into the role of aminopeptidases in the treatment for both preeclampsia and preterm labor

Both aminopeptidase A and placental leucine aminOPEptidases could be potentially safe and effective drugs for patients and their babies in the treatment of preeclampsia and preterm labor.

Maternal serum placental leucine aminopeptidase (P‐LAP)/oxytocinase and preterm delivery

A new approach regarding the treatment of preeclampsia and preterm labor.

Characterization of a secretase activity for placental leucine aminopeptidase.

The results suggest that P-LAP secretase has a metalloprotease activity which depends on the amino acid sequence of the cleavage site.

Oxytocin and Oxytocinase in the Obese and Nonobese Parturients during Induction and Augmentation of Labor

Preliminary evidence that the disposition of OXT may not be different between obese and nonobese women during labor is provided.

Beta-homo-amino acid scan of angiotensin IV.

The identification of H-( R)beta (2)hVal-Tyr-Ile-His-Pro-beta (3)hPhe-OH as a potent and stable Ang IV analog with high selectivity for IRAP versus AP-N and the AT1 receptor is identified.



Immunologic inactivation of human pregnancy serum oxytocinase activity.

Degradation of oxytocin by the human placenta: effect of selective inhibitors.

The results strongly suggested the existence of oxytocinase(s) other than the above three enzymes in microsomal and/or lysosomal fractions of human placenta.

In vitro degradation of oxytocin by pregnancy serum, placental subcellular fractions and purified placental aminopeptidases.

The degradation of oxytocin by human placental particulate and soluble fractions, pregnant and non-pregnant sera, and purified enzymes, and the combination of the post-proline endopeptidase with porcine kidney LAP or with placental microsomal P-LAP actively liberated all amino acids and amides detectable by HPLC.

The distribution of enzymes destroying oxytocin and vasopressin in human placentae

The nature of the destruction of oxytocin and vasopressin by homogenate of human placentae is established and whether or not there were present inplacentae two separate systems each selectively destroying these posterior pituitary hormones is shown.

Structure and expression of a human oxytocin receptor

The structure and expression of the human oxytocin receptor complementary DNA isolated by expression cloning are reported and it is concluded that the increase in receptor number in the myometrium at labour is, at least in part, due to the increased in mRNA.

The value of plasma pitocinase determinations in obstetrics.

  • E. Page
  • Medicine
    American journal of obstetrics and gynecology
  • 1946

Oxytocin and bovine parturition: a steep rise in endometrial oxytocin receptors precedes onset of labor.

The regulation of oxytocin receptors by ovarian hormones in the two tissues may therefore differ, and the regulation of Oxytocin receptor concentrations in the three tissues may also differ.