Oxygenated complex of cytochrome bd from Escherichia coli: Stability and photolability

  title={Oxygenated complex of cytochrome bd from Escherichia coli: Stability and photolability},
  author={I. Belevich and V. Borisov and A. Konstantinov and Michael I. Verkhovsky},
  journal={FEBS Letters},
Cytochrome bd is one of the two terminal ubiquinol oxidases in the respiratory chain of Escherichia coli catalyzing reduction of O2 to H2O. The enzyme is expressed under low oxygen tension; due to high affinity for O2 it is isolated mainly as a stable oxygenated complex. Direct measurement of O2 binding to heme d in the one‐electron reduced isolated enzyme gives K d(O2) of ∼280 nM. It is possible to photolyse the heme d oxy‐complex by illumination of the enzyme for several minutes under… Expand
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