Oxygen activation in neuronal NO synthase: resolving the consecutive mono-oxygenation steps.

@article{Papale2012OxygenAI,
  title={Oxygen activation in neuronal NO synthase: resolving the consecutive mono-oxygenation steps.},
  author={Davide Papale and Chiara Bruckmann and Ben Gazur and Caroline S. Miles and Christopher G. Mowat and Simon N Daff},
  journal={The Biochemical journal},
  year={2012},
  volume={443 2},
  pages={505-14}
}
The vital signalling molecule NO is produced by mammalian NOS (nitric oxide synthase) enzymes in two steps. L-arginine is converted into NOHA (Nω-hydroxy-L-arginine), which is converted into NO and citrulline. Both steps are thought to proceed via similar mechanisms in which the cofactor BH4 (tetrahydrobiopterin) activates dioxygen at the haem site by electron transfer. The subsequent events are poorly understood due to the lack of stable intermediates. By analogy with cytochrome P450, a haem… CONTINUE READING