Oxyanion and tetrahedral intermediate stabilisation by subtilisin: detection of a new tetrahedral adduct.

@article{Howe2009OxyanionAT,
  title={Oxyanion and tetrahedral intermediate stabilisation by subtilisin: detection of a new tetrahedral adduct.},
  author={Nicole Howe and Louis Rogers and Chandralal M. Hewage and J. Paul G. Malthouse},
  journal={Biochimica et biophysica acta},
  year={2009},
  volume={1794 8},
  pages={1251-8}
}
The peptide-derived glyoxal inhibitor Z-Ala-Ala-Phe-glyoxal has been shown to be approximately 10 fold more effective as an inhibitor of subtilisin than Z-Ala-Pro-Phe-glyoxal. Signals at 107.2 ppm and 200.5 ppm are observed for the glyoxal keto and aldehyde carbons of the inhibitor bound to subtilisin, showing that the glyoxal keto and aldehyde carbons are sp(3) and sp(2) hybridised respectively. The signal at 107.2 ppm from the carbon atom attached to the hemiketal oxyanion is formed in a slow… CONTINUE READING