Oxidized messenger RNA induces translation errors.

  title={Oxidized messenger RNA induces translation errors.},
  author={Mikiei Tanaka and P. Boon Chock and Earl R. Stadtman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={104 1},
To investigate the effect of RNA oxidation on normal cellular functions, we studied the translation of nonoxidized and oxidized luciferase mRNA in both rabbit reticulocyte lysate and human HEK293 cells. When HEK293 cells transfected with nonoxidized mRNA encoding the firefly luciferase protein were cultured in the presence of paraquat, there was a paraquat concentration-dependent increase in the formation of luciferase short polypeptides (SPs) concomitant with an increase in 8-oxoguanosine… CONTINUE READING