Oxidatively generated base damage to cellular DNA.

  title={Oxidatively generated base damage to cellular DNA.},
  author={Jean Cadet and Thierry Douki and J L Ravanat},
  journal={Free radical biology & medicine},
  volume={49 1},
Search for the formation of oxidatively base damage in cellular DNA has been a matter of debate for more than 40 years due to the lack of accurate methods for the measurement of the lesions. HPLC associated with either tandem mass spectrometry (MS/MS) or electrochemical detector (ECD) together with optimized DNA extraction conditions constitutes a relevant analytical approach. This has allowed the accurate measurement of oxidatively generated single and clustered base damage in cellular DNA… CONTINUE READING