Oxidative stress measurement by in vivo electron spin resonance spectroscopy in rats with streptozotocin-induced diabetes

@article{Sano1998OxidativeSM,
  title={Oxidative stress measurement by in vivo electron spin resonance spectroscopy in rats with streptozotocin-induced diabetes},
  author={Takahisa Sano and Fumio Umeda and Toshihiko Hashimoto and Hajime Nawata and Hideo Utsumi},
  journal={Diabetologia},
  year={1998},
  volume={41},
  pages={1355-1360}
}
Enhanced oxidative stress in diabetic patients may contribute to the pathogenesis of diabetic angiopathy. We have recently developed a method to determine the electron spin resonance (ESR, electron paramagnetic resonance; EPR) of reactive oxygen species and free radicals in vivo, using the nitroxide derivative, carbamoyl-PROXYL as a probe. In this study, diabetes was induced in Wistar rats by streptozotocin (STZ) injection (65 mg/kg, body weight, intravenously). Two, 4, and 8 weeks later, the… CONTINUE READING