Oxidative stress induces S-thiolation of specific proteins in cultured gastric mucosal cells.

@article{Rokutan1994OxidativeSI,
  title={Oxidative stress induces S-thiolation of specific proteins in cultured gastric mucosal cells.},
  author={Kazuhito Rokutan and Richard B. Johnston and Keiichi Kawai},
  journal={The American journal of physiology},
  year={1994},
  volume={266 2 Pt 1},
  pages={G247-54}
}
Oxidative stress induces the formation of protein-mixed disulfides with low-molecular-weight thiols, especially glutathione. We analyzed this process, termed S-thiolation, in cultured gastric mucosal cells from guinea pigs by gel electrophoresis and autoradiography after radiolabeling of the intracellular glutathione pool with 35S. Hydrogen peroxide (H2O2) or diamide initiated rapid and reversible S-thiolation of specific proteins with molecular masses of 42, 30, 29, 28, and 22 kDa. Diamide… CONTINUE READING

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