Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca2+ and includes lipid peroxidation and alterations in membrane proteins.

Abstract

Isolated rat liver mitochondria exposed to Fe(II)citrate undergo lipid peroxidation and alterations in membrane proteins. These processes were associated with irreversible decrease in membrane potential and mitochondrial swelling. Lipid peroxidation was evidenced by the production of thiobarbituric acid-reactive substances and also by the reaction of these products with membrane proteins, through the formation of Schiff bases. Alterations in membrane proteins were also characterized by the loss of specific proteins that could be recovered from the mitochondrial supernatant as shown by SDS-polyacrylamide gel electrophoresis. The degree of both lipid peroxidation and alterations in membrane proteins were diminished by EGTA, ruthenium red, or dibucaine. This strongly indicates that Ca2+ potentiates the oxidative damage of mitochondria exposed to Fe(II)citrate.

0200400'96'98'00'02'04'06'08'10'12'14'16
Citations per Year

791 Citations

Semantic Scholar estimates that this publication has 791 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Castilho1994OxidativeDO, title={Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca2+ and includes lipid peroxidation and alterations in membrane proteins.}, author={Roger F Castilho and Andr{\'e} R. Meinicke and Andr{\'e}a Miyasaka Almeida and Marcelo Hermes-Lima and Anibal E Vercesi}, journal={Archives of biochemistry and biophysics}, year={1994}, volume={308 1}, pages={158-63} }