Oxidative Stress EPR Measurement in Human Liver by Radical-probe Technique. Correlation with Etiology, Histology and Cell Proliferation

@article{Valgimigli2002OxidativeSE,
  title={Oxidative Stress EPR Measurement in Human Liver by Radical-probe Technique. Correlation with Etiology, Histology and Cell Proliferation},
  author={Marco Valgimigli and Luca Valgimigli and Davide Trer{\'e} and Stefano Gaiani and Gian Franco Pedulli and Laura Gramantieri and Luigi Bolondi},
  journal={Free Radical Research},
  year={2002},
  volume={36},
  pages={939 - 948}
}
The role of reactive oxygen species (ROS) in liver disease is controversial. This mostly reflects the difficulties to quantify ROS in vivo, particularly in humans. We aimed to measure the presence of ROS in diseased human liver and identify possible relations between ROS levels and etiology, histology and hepatocyte proliferation. Liver biopsy specimens from 102 individuals: 18 healthy controls and 84 patients (42 HCV chronic hepatitis (CHC), 19 HBV chronic hepatitis (CHB), 7 PBC, 4 PSC, 4 HCV… Expand
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References

SHOWING 1-10 OF 70 REFERENCES
Measurement of oxidative stress in human liver by EPR spin-probe technique
TLDR
The method is based on the EPR detection of the nitroxide radical produced by reaction of the hydroxylamine spin-probe with ROS generated under pseudo-physiologic conditions in fine needle biopsies of healthy and diseased human liver. Expand
Association between reactive oxygen species and disease activity in chronic hepatitis C.
TLDR
Data demonstrate that lipid and protein oxidation occur in chronic hepatitis C, oxidative damage can be demonstrated as increased serum levels of MDA and PCC, and both MDAand PCC levels correlate with disease activity. Expand
Hepatocyte proliferative activity in chronic liver damage as assessed by the monoclonal antibody MIB1 Ki67 in archival material: The role of etiology, disease activity, iron, and lipid peroxidation
TLDR
The data suggest that HCV infection induces increased and abnormal hepatocyte proliferation, which might be related to the increased risk of hepatocellular carcinoma in patients with HCV‐related liver damage. Expand
Iron storage, lipid peroxidation and glutathione turnover in chronic anti-HCV positive hepatitis.
TLDR
Findings suggest that hepatitis C virus-related liver damage is characterized by increased iron storage (possibly induced by the virus) which elicits a free-radical-mediated peroxidation, with consequent steatosis and activation of glutathione turnover, probably in response to free- radical-mediated liver damage. Expand
In situ detection of lipid peroxidation by‐products in chronic liver diseases
TLDR
Immunohistochemical results suggest that lipid peroxidation by‐products are detectable in chronic liver diseases, and that this mechanism is implicated very early in the pathogenesis of some of these diseases. Expand
Reduced glutathione concentration in erythrocytes of patients with acute and chronic viral hepatitis
TLDR
Evaluating the glutathione concentration in erythrocytes in patients with acute and chronic viral hepatitis found that the mean value of GSH was below the normal range, with high alanine aminotransferase (ALT) activity not significantly different in groups with chronic HBV and HCV infection. Expand
Elevated level of serum Mn-superoxide dismutase in patients with primary biliary cirrhosis: possible involvement of free radicals in the pathogenesis in primary biliary cirrhosis.
TLDR
Serum Mn-superoxide dismutase was determined in patients with various liver diseases and suggested that free radicals including superoxide anion are possibly involved in the pathogenesis of the disease and Mn-SOD may play some role in a protection against the superoxideAnion. Expand
Superoxide dismutase in patients with chronic hepatitis C virus infection.
TLDR
The results indicate that in HCV infection an induction of Mn-SOD was present in PBMC but absent in the liver, suggesting that this organ could be less protected against oxidative damage. Expand
Oxidative stress in patients with hepatitis, cirrhosis, and hepatoma evaluated by plasma antioxidants.
TLDR
Results and a significant decrease in the plasma ascorbate level in patient groups indicate that oxidative stress is evident after the onset of hepatitis and the subsequent cirrhosis and liver cancer. Expand
Extensive oxidative DNA damage in hepatocytes of transgenic mice with chronic active hepatitis destined to develop hepatocellular carcinoma.
  • T. Hagen, S. Huang, +5 authors F. Chisari
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1994
TLDR
HBV transgenic mice with chronic active hepatitis display greatly increased hepatic oxidative DNA damage, which occurs in the presence of heightened hepatocellular proliferation, increasing the probability of fixation of the attendant genetic and chromosomal abnormalities and the development of hepato cellular carcinoma. Expand
...
1
2
3
4
5
...