Oxidative Stress–Induced Degradation of Thioredoxin-1 and Apoptosis Is Inhibited by Thioredoxin-1–Actin Interaction in Endothelial Cells

@article{Zschauer2011OxidativeSD,
  title={Oxidative Stress–Induced Degradation of Thioredoxin-1 and Apoptosis Is Inhibited by Thioredoxin-1–Actin Interaction in Endothelial Cells},
  author={Tim-Christian Zschauer and Kerstin Kunze and Sascha M. Jakob and Judith Haendeler and Joachim Altschmied},
  journal={Arteriosclerosis, Thrombosis, and Vascular Biology},
  year={2011},
  volume={31},
  pages={650-656}
}
Objective—Thioredoxin-1 (Trx-1), one important antioxidative enzyme in endothelial cells, is required for apoptosis inhibition. Apoptosis induction is dependent on cytoskeletal changes, which depend on actin rearrangements. Therefore, we wanted to elucidate whether a physical interaction exists between Trx-1 and actin and what the functional consequences are. Methods and Results—Combined immunoprecipitation/mass spectrometry identified actin as a new binding partner for Trx-1. A separate pool… 
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