Oxidations by Erythrocytes and the Catalytic Influence of Methylene Blue

Abstract

The marked increase of respiration and the accompanying oxidation of carbohydrate exhibited by mammalian erythrocytes under the catalytic influence of methylene blue (Harrop and Barron (1)) make this semibiological model an instructive one for the study both of carbohydrate oxidation and the catalytic factors essential to respiration. In a preceding paper (2) we reported experiments which show that of the carbohydrate oxidized by this system one part is lactic acid, while another part is some other glucose derivative or, perhaps, the sugar itself. The purpose of the present paper is to present experiments which concern especially the mechanism of the catalysis by methylene blue in this system. Because fairly accurate chemical methods became available for determination of both substrate and product, we chose the less complex oxidation of lactate to pyruvate (in the absence of glucose) as the reaction with which to study the mechanism of the catalytic factors involved. As explained in Paper I, it was early found (3) that the methemoglobin is formed in the course of the dye catalysis. In several of our first experiments a constant relation-not confirmed in later work-appeared to exist between the amounts of pyruvate and of methemoglobin formed. In attempting to relate these oxidations to the reversible reactions of the dye, we were led astray by this

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Cite this paper

@inproceedings{Wendel2003OxidationsBE, title={Oxidations by Erythrocytes and the Catalytic Influence of Methylene Blue}, author={W. Bradley Wendel}, year={2003} }