Oxidation of the carbanion intermediate of transaldolase by hexacyanoferrate (III).

  title={Oxidation of the carbanion intermediate of transaldolase by hexacyanoferrate (III).},
  author={Philipp Christen and Anna Gasser},
  journal={The Journal of biological chemistry},
  volume={251 14},
The transaldolase-dihydroxyacetone carbanion intermediate formed in the reaction of transaldolase with its donor substrates fructose-6-P or sedoheptulose-7-P is susceptible to oxidation by hexacyanoferrate(III). The dihydroxyacetone moiety is oxidized to the corresponding 2-ketoaldehyde, i.e. hydroxy-pyruvaldehyde (CH2OH-CO-CHO). This oxidation product is, in contrast to dihydroxyacetone, readily released from the enzyme. In the presence of hexacyanoferrate(III) transaldolase thus functions as… Expand
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