Oxidation of methionine residues in proteins of activated human neutrophils.

  title={Oxidation of methionine residues in proteins of activated human neutrophils.},
  author={Henry Fliss and Herbert Weissbach and Nathan Brot},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={80 23},
A simple assay for the detection of 35S-labeled methionine sulfoxide residues in proteins is described. The assay, which is based on the ability of CNBr to react with methionine but not with methionine sulfoxide, requires the prelabeling of cellular proteins with [35S]methionine. The assay was used to study the extent of methionine oxidation in newly synthesized proteins of both activated and quiescent human neutrophils. In cells undergoing a phorbol 12-myristate 13-acetate-induced respiratory… CONTINUE READING
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