Oxidation of glycerol to formaldehyde by rat liver microsomes.

  title={Oxidation of glycerol to formaldehyde by rat liver microsomes.},
  author={Debra K. Winters and Liviu Clejan and Arthur I. Cederbaum},
  journal={Biochemical and biophysical research communications},
  volume={153 2},

Oxidation of tris to one-carbon compounds in a radical-producing model system, in microsomes, in hepatocytes and in rats.

The results indicate that an oxidant formed via a Fenton-type reaction, possibly the hydroxyl radical, may be involved in the formation of one-carbon compounds from Tris.

Ethanol-inducible cytochrome P-450: assessment of substrates' specific chemical probes in rat liver microsomes.

Results suggest that NDMA and especially 1-butanol are the most specific and useful probes of alcohol-inducible cytochrome P-450 in crude liver microsomes in rats treated with different xenobiotics.

Recognition of novel artifacts produced during the microsomal incubation of secondary alicyclic amines in the presence of cyanide.

  • J. GorrodY. Sai
  • Biology, Chemistry
    Xenobiotica; the fate of foreign compounds in biological systems
  • 1997
The results indicate that care should be taken in interpreting data obtained from incubates containing secondary amines and cyanide, especially in the presence of substrates capable of donating formaldehyde, in direct relationship to the amount of formaldehyde generated.

Cytochrome P450 isozymes responsible for the metabolism of toluene and styrene in human liver microsomes.

It is suggested that CYP2E1 and CYP1A2/1 are the main isoforms responsible for the metabolism of toluene at low substrate concentrations in human liver microsomes, CYP 2E1 at low styrene concentration, and CyP2C8 and CYB2B6 at high concentrations of toLUene and styrene respectively.

Oxidative and reductive metabolism by cytochrome P450 2E1

  • D. Koop
  • Biology, Chemistry
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1992
The enzyme efficiently catalyzes the low Km metabolism of compounds commonly used as solvents in industry and at home as well as components found in cigarette smoke, many of which are established carcinogens and hepatotoxins.

Function, expression and polymorphism of human alcohol dehydrogenase 3/glutathionedependent formaldehyde dehydrogenase

Substantial capacity for formaldehyde detoxification was shown from quantitative assessments of alcohol and aldehyde oxidizing activities including Km-determinations, demonstrating that ADH3 is the major enzyme involved in formaldehyde oxidation in oral mucosa.

Expression, Catalytic Activity, and Inducibility of Cytochrome P450 2E1 (CYP2E1) in the Rat Central Nervous System

It is concluded that CyP2E1 is expressed and catalytically active in the rat CNS, and that CYP2 E1 can be induced by a relatively low concentration of ethanol in cortical glial cultures.



Stereochemical studies on the cytochrome P-450 and hydroxyl radical dependent pathways of 2-butanol oxidation by microsomes from chow-fed, phenobarbital-treated, and ethanol-treated rats.

2-Butanol appears to be a particularly good alcohol substrate for the cytochrome P-450 dependent pathway of alcohol oxidation and Chronic ethanol consumption, which induces the microsomal alcohol oxidizing system, resulted in a 3-fold increase in the rate of 2-butanol oxidation.


The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.

Thermodynamic and kinetic examination of protein stabilization by glycerol.

Results of the kinetic examination of the slow unfolding reaction are consistent with the concept that the preferential exclusion of glycerol is related, at least in part, to enhanced solvent ordering.

Cytochrome P-450: Structure, Mechanism, and Biochemistry

The Cytochrome P450 and the Metabolism and Bioactivation of Arachidonic Acid and Eicosanoids in Plants and the Diversity and Importance of Microbial Cytochromes P450 are studied.

The colorimetric estimation of formaldehyde by means of the Hantzsch reaction.

  • T. Nash
  • Chemistry, Medicine
    The Biochemical journal
  • 1953
A chronology of key events leading up to and including the birth of Bonnichsen, R. K. & Bonner, J. (1952).