Oxidation of glucosamine by human polymorphonuclear leukocytes

  title={Oxidation of glucosamine by human polymorphonuclear leukocytes},
  author={C L Swendsen and Lawrence R. Dechatelet},
When exposed to a phagocytic stimulus (opsonized zymosan), human polymorphonuclear leukocytes (PMNs) produced14CO2 from [1-14C]glucosamine at a rate 10–25% of that produced from glucose under the same conditions. The production of CO2 from glucosamine by intact PMNs was inhibited by glucose and dependent upon activation of the hexosemonophosphate shunt (HMPS). However, the metabolic pathways for the oxidation of glucose and glucosamine by PMNs are not identical. This is suggested by the fact… Expand


Effect of phorbol myristate acetate on the oxidative metabolism of human polymorphonuclear leukocytes.
Results suggest that PMA activates the cell in the same manner as does phagocytosis, and this compound should provide a useful tool for elucidating the metabolic events underlying the phenomena of phagcytosis and bacterial killing by polymorphonuclear leukocytes. Expand
Catabolism of 2-deoxyglucose by phagocytic leukocytes in the presence of 12-O-tetradecanoyl phorbol-13-acetate.
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  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1978
2-deoxyglucose decarboxylation should be considered whenever this analog of mannose and glucose is used as an indicator for sugar transport, especially when pharmacodynamic agents are present. Expand
Glucose-6-phosphate Dehydrogenase
Publisher Summary This chapter discusses glucose-6-phosphate dehydrogenase (G6P-DH), which was first isolated from erythrocytes and from fermenting yeast by Warburg et al., who carried out anExpand
Stimulation of Leukocyte Hexose Monophosphate Shunt Activity by Ascorbic Acid
Ascorbic acid in vitro markedly stimulates the hexose monophosphate shunt in normal human neutrophils, rabbit alveolar macrophages, and in neutrophils from a patient with chronic granulomatousExpand
Chloramines as intermediates of oxidation reaction of amino acids by myeloperoxidase.
The mechanism of the deamination-decarboxylation of amino acids by myeloperoxidase is proposed and it is shown that amino acids as unstable compounds are spontaneously decomposed to NH 3, CO 2, Cl − and corresponding aldehydes. Expand
[12] Colorimetric analysis of sugars
Publisher Summary This chapter presents a procedure for the calorimetric analysis of sugars for the identification and determination of small quantities of carbohydrates obtained from biologicalExpand
A modified procedure for the determination of leukocyte alkaline phosphatase.
A satisfactory assay for the leukocyte enzyme employing 2-amino-2-methyl-1-propanol buffer is outlined, and the reaction is not linear with time when run in this buffer. Expand
Determination of submicro quantities of ammonia.
  • A. Levitzki
  • Chemistry, Medicine
  • Analytical biochemistry
  • 1970
The method makes use of glutamic dehydrogenase as a specific ammonia reagent when applied to the reductive amination of α-ketoglutaric acid to determine submicro quantities of ammonia. Expand
[Glucose-6-phosphate dehydrogenase].
The metabolic burst. In The Neutrophil: Functional and Clinical Disorders
  • 1978