Oxidation of NADH by vanadate plus phenylmethanesulfonyl fluoride (PMSF).

@article{Takeda1988OxidationON,
  title={Oxidation of NADH by vanadate plus phenylmethanesulfonyl fluoride (PMSF).},
  author={Kazuo Takeda and Masaru Kawamura},
  journal={Journal of UOEH},
  year={1988},
  volume={10 1},
  pages={59-62}
}
Phenylmethanesulfonyl fluoride (PMSF) enabled vanadate to catalyze the oxidation of NADH. Superoxide dismutase and manganese inhibited the oxidation, suggesting that superoxide anion played a crucial role. Since PMSF is widely used to protect enzymes from proteolytic cleavage when they are purified, attention should be paid when the toxicity of vanadate on… CONTINUE READING