Oxidation catalyzed by H+ ions improves the silver intensification of 3,3′-diaminobenzidine staining by strongly suppressing tissue argyrophilia

Abstract

We found that a 15-min treatment of tissue sections with 2 M/l H2O2 dissolved in 6 M/l sulphuric acid strongly suppresses the argyrophilia of tissue elements, thus lacilitating selective silver intensification in histochemical procedures. When applied inserted between the 3,3′-diaminobenzidine reaction and physical development, this treatment allows the… (More)
DOI: 10.1007/BF00492479

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