Oxidants and antioxidants in alveolar epithelial type II cells: in situ, freshly isolated, and cultured cells.

@article{Kinnula1992OxidantsAA,
  title={Oxidants and antioxidants in alveolar epithelial type II cells: in situ, freshly isolated, and cultured cells.},
  author={Vuokko L. Kinnula and Ling Yi Chang and Jeffrey I Everitt and James D. Crapo},
  journal={The American journal of physiology},
  year={1992},
  volume={262 1 Pt 1},
  pages={L69-77}
}
Antioxidant enzyme activities, H2O2 clearance, and H2O2 generation by rat alveolar epithelial type II cells were compared between in situ, freshly isolated (6 h ex vivo), and cultured cells (48 h ex vivo). Immunocytochemical studies did not show changes in catalase, Mn superoxide dismutase, or CuZn superoxide dismutase labeling density in cytoplasm, peroxisomes, or mitochondria. Numbers of peroxisomes and mitochondria per cell decreased in cultured cells. Biochemical studies showed that cell… CONTINUE READING

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