Ovine-specific Y-chromosome RAPD-SCAR marker for embryo sexing.

  title={Ovine-specific Y-chromosome RAPD-SCAR marker for embryo sexing.},
  author={Alfonso Guti{\'e}rrez-Ad{\'a}n and W. T. Cushwa and Gary B. Anderson and Juan F Medrano},
  journal={Animal genetics},
  volume={28 2},
An accurate, sensitive, and quick (approximately 3 h) method for determining the sex of ovine embryos was developed using polymerase chain reaction (PCR) primers derived from an ovine-specific Y-chromosome random amplified polymorphic DNA marker (UcdO43). The accuracy and sensitivity of the assay were first tested using genomic DNA from 10 males and 10 females of five different sheep breeds, and then tested using serial dilutions of male-in-female DNA. The assay was 100% accurate in confirming… CONTINUE READING

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