Overview of very small embryonic-like stem cells (VSELs) and methodology of their identification and isolation by flow cytometric methods.

@article{ZubaSurma2010OverviewOV,
  title={Overview of very small embryonic-like stem cells (VSELs) and methodology of their identification and isolation by flow cytometric methods.},
  author={Ewa K. Zuba-Surma and Mariusz Z Ratajczak},
  journal={Current protocols in cytometry},
  year={2010},
  volume={Chapter 9},
  pages={Unit9.29}
}
The protocols presented here describe the procedures employed to identify and isolate very small embryonic-like stem cells (VSELs) using flow cytometric technologies including fluorescence-activated cell sorting (FACS). We describe the recommended steps in detail for their successful identification and isolation from adult tissues. These protocols were initially established to isolate such cells from murine bone marrow (BM) and human cord blood (CB) and may also be employed to isolate these… CONTINUE READING

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Plasticity of adult stem cells

  • W. Wojakowski, M. Tendera, +11 authors M. Z. Ratajczak
  • 2009
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