Overview of the Retrovirus Transduction System

  title={Overview of the Retrovirus Transduction System},
  author={C. Cepko and W. Pear},
  journal={Current Protocols in Molecular Biology},
  • C. Cepko, W. Pear
  • Published 2001
  • Biology, Medicine
  • Current Protocols in Molecular Biology
A retrovirus vector is an infectious virus used to introduce a nonviral gene into mitotic cells in vivo or in vitro. The efficient and precise integration machinery of naturally occurring retroviruses is utilized to produce either a single copy or a few copies of the viral genome stably integrated into a host chromosome. This unit presents an overview of the retrovirus life cycle and a description of vector designs and packaging cell lines. 
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