Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

  title={Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems},
  author={K. Terpe},
  journal={Applied Microbiology and Biotechnology},
  • K. Terpe
  • Published 2002
  • Biology, Medicine
  • Applied Microbiology and Biotechnology
  • Abstract. In response to the rapidly growing field of proteomics, the use of recombinant proteins has increased greatly in recent years. Recombinant hybrids containing a polypeptide fusion partner, termed affinity tag, to facilitate the purification of the target polypeptides are widely used. Many different proteins, domains, or peptides can be fused with the target protein. The advantages of using fusion proteins to facilitate purification and detection of recombinant proteins are well… CONTINUE READING
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